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DOI :
10.3791/59350-v
•
10:18 min
May 28th, 2019
Chapters
0:04
Title
1:22
Single CRISPR RNA (crRNA) Array Expression Cassette
2:10
S. cerevisiae Transformation
5:02
Second Transformation
6:42
Genome Editing Efficiency Evaluation and Yeast Pixel Art Creation
8:30
Results: Transformation Verification and Yeast Pixel Art Creation
9:39
Conclusion
단일 crRNA 어레이와 결합된 CRISPR/Cas12a 시스템은 여러 로시에서 동시에 S. 세레비시아 게놈의 효율적인 멀티플렉스 편집을 가능하게 합니다. 이것은 이후에 효모 픽셀 예술을 만드는 데 사용되는 효모 균주를 생산하는 카로티노이드를 구성하여 입증됩니다.
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