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DOI :
10.3791/59384-v
•
13:34 min
April 16th, 2019
Chapters
0:04
Title
2:12
Preparation of Astrocytes
4:08
Preparation of Endothelial Cells
6:10
Preparation of Glioma Cells
7:43
Measurement of the BBTB Mimic Permeability (Optional)
8:48
BBTB Fluorescence Staining to Detect Nanoparticle Transcytosis
10:08
Results: Analysis of In Vivo Payload Delivery using the Blood-brain Tumor-barrier
12:25
Conclusion
中枢神経系腫瘍にターゲティングは大きな課題です。ここで我々 はマウスやヒトの細胞を用いた血液脳腫瘍関門の in vitro における模倣を生産し、中枢神経系腫瘍生体内ターゲットの予測可能性の重要性を議論するためのプロトコルについて説明します。
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