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DOI :
10.3791/59427-v
•
7:21 min
June 23rd, 2019
Chapters
0:04
Title
1:02
Bronchoalveolar (BAL) Cell Isolation
2:33
Whole BAL Cell and Peripheral Blood Mononuclear Cell (PBMC) Sorting
5:48
Results: Representative Alveolar Macrophage Immunophenotyping
6:41
Conclusion
我们描述了一种处理支气管性肠液的方法,以及通过抗逆转录病毒疗法对慢性艾滋病毒感染者进行匹配的外周血,以评估肺艾滋病毒储存。这些方法导致获得高度纯的CD4 T细胞和海泡巨噬细胞,随后可用于免疫分型和HIV DNA/RNA定量通过超敏感聚合酶链反应。
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