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DOI :
10.3791/59428-v
May 2nd, 2019
Chapters
0:04
Title
1:37
RNA Extraction and Reverse Transcription of the Viral RNA
3:08
First-round PCR
4:03
Second-round PCR
4:41
Analysis of the PCR Products by Electrophoresis on Agarose Gels
5:48
Results: Evaluation of the Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
7:44
Conclusion
汎オーストラリアコウモリリッサウイルス入れ子逆転写ポリメラーゼ連鎖反応は、すべての既知の lyssaviruses を特異的に検出するために開発されました。異なる動物種の狂犬病脳サンプルを用いたバリデーションは、この方法が、金標準蛍光抗体検査と同等の感受性および特異性を有し、日常的な狂犬病診断に使用できることを示した。
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