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DOI :
10.3791/59446-v
April 23rd, 2019
Chapters
0:04
Title
0:42
Hana3A Cells Culture
2:26
Preparation of the Cells for Transfection
3:23
Plasmid Transfection
5:01
Substrate Incubation
6:07
Odorant Stimulation and Data Analysis
8:07
Results: Real-time Activation and Dose Dependent Responses
9:21
Conclusion
生理的には、嗅覚受容体は匂い分子気相に吸入によってアクティブ化されます。ただし、ほとんどの in vitro システムは、液相嗅覚刺激を利用します。嗅覚刺激による気相匂い物質受容体活性化のリアルタイムでの in vitro の監視を可能にする方法を紹介します。
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