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DOI :
10.3791/59452-v
July 10th, 2019
Chapters
0:04
Title
1:13
hiPSC-CMs Purification, Small Molecule Pre-treatment, Myocardial Infarction, and Cell Transplantation
3:42
Calcium Transient and Contractility Recording
5:21
Results: Increased Engraftment of Transplanted hiPSC-CM Preconditioned with the ROCK Inhibitor Y-27632 in a Murine MI Model
7:11
Conclusion
이 프로토콜에서, 우리는 심근 세포 분화 및 정제를 위해 인간 유도 만능 줄기 세포를 사용하는 방법에 대해 설명하고 정교하게, 그리고 더 나아가, Rho 관련 단백질 키나아제 억제제와 함께 이식 효율을 개선하는 방법에 대해 마우스 심근 경색 모델에서 전처리.
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