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DOI :
10.3791/59462-v
June 13th, 2019
Chapters
0:04
Title
1:10
RNA Isolation by Guanidinium Thiocyanate-phenol Extraction and cDNA Library Generation by Reverse Transcription
4:35
Mitochondrial Gene Amplification, Purification, Sequencing and Comparison
7:27
Applying the cDNA Library for Cloning the Genes MgUGD and MgUXS
8:42
Expression and Activity Tests of MgUGD and MgUXS
10:32
Results: Sequencing and Mass Spectrometry Results
11:33
Conclusion
우리는 참조되지 않은 태평양 굴 견본에서 RNA 견본을 사용하는 방법에 대한 전략을 기술하고, 사실상 연속된 cDNA 라이브러리를 생성하기 위하여 공개적으로 유효한 게놈 데이터와 비교하여 유전 물질을 평가합니다.
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