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DOI :
10.3791/59495-v
•
8:52 min
March 14th, 2019
Chapters
0:04
Title
0:33
Preparation of V. natriegens Crude Cell Extracts Bacterial Culture
1:48
Preparation of V. natriegens Crude Cell Extracts Cell Lysis
4:25
Cell-free Protein Expression Reactions Using V. natriegens Crude Extract
7:06
Results: Protein Yield Using Cell-free Protein Expression System with Vibrio natriegens
8:02
Conclusion
无细胞表达系统是用于高吞吐量合成和筛选重要蛋白质的功能强大且经济高效的工具。在这里, 我们描述了使用纳利根弧菌快速生产的无细胞蛋白表达系统的制备, 使用质粒 dna, 线性 dna 和 mrna 模板。
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