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DOI :
10.3791/59495-v
•
8:52 min
March 14th, 2019
Chapters
0:04
Title
0:33
Preparation of V. natriegens Crude Cell Extracts Bacterial Culture
1:48
Preparation of V. natriegens Crude Cell Extracts Cell Lysis
4:25
Cell-free Protein Expression Reactions Using V. natriegens Crude Extract
7:06
Results: Protein Yield Using Cell-free Protein Expression System with Vibrio natriegens
8:02
Conclusion
無細胞発現系は、ハイスループット合成と重要なタンパク質のスクリーニングのための強力かつコスト効率の高いツールです。ここで、mRNA のテンプレート、線形 DNA プラスミド DNA を使用して急速な蛋白質の生産のビブリオ natriegensを用いた無細胞タンパク質発現系の作製について述べる。
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