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DOI :
10.3791/59526-v
June 7th, 2019
Chapters
0:04
Title
1:06
Motorized Treadmill Construction
3:22
In Vivo Two-photon Fluorescence Lifetime Imaging (2pFLIM) Microscopy
6:29
2pFLIM Image Analysis
8:38
Results: Representative 2pFLIM Data Quantification and Targeted A-Kinase Activity-reporter-alpha (t-AKAR-alpha) Tracking
9:29
Conclusion
提出了一个程序,以可视化蛋白激酶A活动在头部固定,行为小鼠。改进的A-激酶活动报告器tAKAR®在皮质神经元中表达,并通过颅窗口进行成像。双光子荧光寿命成像显微镜用于在强制运动期间可视化体内的PKA活动。
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