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DOI :
10.3791/59527-v
•
9:07 min
May 1st, 2019
Chapters
0:04
Title
0:53
Inoculum Preparation
1:34
Growth and Harvesting of Mycelia
2:36
Preparation of the Protein Extract and Equilibration of the IgG Resin
4:40
Batch Purification of TAP-tagged HDAC
6:50
Results: Analysis of the Purification and Enzymatic Activity Assay
8:29
Conclusion
クラス1のヒストンデアセチラーゼ (HDACs) RpdA のような真菌感染症を治療する潜在的なターゲットとして重要性を得ています。.ここでは、ヒストンデアセチラーゼ阻害剤のインビトロ薬効試験を可能にする HDAC 活性アッセイと組み合わせた、TAP タグ付き RpdA の特異的な濃縮のためのプロトコルを提示する。
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