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DOI :
10.3791/59537-v
•
8:10 min
June 24th, 2019
Chapters
0:04
Title
1:09
Construction of a ZIKV Infectious cDNA Clone in a BAC
1:41
Preparation of High-purity pBAC-ZIKV for the Rescue of Infectious rZIKV
2:36
Rescue of Infectious rZIKV from the BAC cDNA Clone by Transfection of Vero Cells
4:40
Amplification and Generation of Viral Stocks
5:58
Results: Analysis of the Rescued Recombinant Zika Virus
7:34
Conclusion
寨卡病毒最近的流行突出表明,必须建立反向遗传方法,以开发疫苗和/或治疗战略。在这里,我们描述了从全长cDNA克隆中拯救传染性重组寨卡病毒的协议,该克隆在人类细胞巨细胞病毒立即-早期启动子的控制下组装在细菌人工染色体中。
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