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DOI :
10.3791/59537-v
•
8:10 min
June 24th, 2019
Chapters
0:04
Title
1:09
Construction of a ZIKV Infectious cDNA Clone in a BAC
1:41
Preparation of High-purity pBAC-ZIKV for the Rescue of Infectious rZIKV
2:36
Rescue of Infectious rZIKV from the BAC cDNA Clone by Transfection of Vero Cells
4:40
Amplification and Generation of Viral Stocks
5:58
Results: Analysis of the Rescued Recombinant Zika Virus
7:34
Conclusion
ジカウイルスの最近の流行は、ワクチンや治療戦略を開発するための逆遺伝的アプローチを確立することの重要性を強調しています。ここでは、ヒトサイトメガロウイルスの制御下で細菌人工染色体に組み立てられた全長cDNAクローンから感染性組換キンジカウイルスを早期に促進するプロトコルについて説明する。
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