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DOI :
10.3791/59555-v
April 30th, 2019
Chapters
0:04
Title
0:31
Lipid-containing Oil Solution and Small Vesicle (SV) Preparation
1:54
Bacterial Cell Pre-culture
2:45
Giant Vesicle (GV) and Water-in-Oil (W/O) Bacterial Cell-containing Droplet Formation
4:11
Handmade Chamber and Supported Bilayer Membrane Preparation
4:54
GV Immobilization and Microscopic Bacterial Growth Observation
6:04
Results: Representative GV Containing Bacteria and Bacterial Growth Evaluation
6:44
Conclusion
우리는 거대한 소포 (GV) 안쪽에 박테리아의 단세포 배양을 보여줍니다. 세균 세포를 함유하는 GV는 액적 전달 방법에 의해 제조되었고, 세균 성장의 직접적인 관찰을 위해 유리 기판 위에 지지막 에 고정시켰다. 이 접근은 또한 그밖 세포에 적응할 수 있습니다.
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