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JoVE Journal

Neuroscience

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Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay

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08:33 min

June 25th, 2019

June 25th, 2019

8,034 Views

0:04

Title

1:17

Tissue Fixation and Storage

2:15

Tissue Cut with a Cryotome

3:11

Immunofluorescence Staining

5:34

Immunofluorescence Imaging

6:01

Results: Analysis of the Free-floating Immunofluorescence Assay

8:09

Conclusion

Transcript

The diagnosis of Parkinson's disease still relies on clinical signs of motor involvement that appear later on in the course of the disease, when most of the neurons of the substantia nigra are already lost. This protocol allows the dermal nerves a

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Here, we present a protocol for a free-floating indirect immunofluorescence assay on skin biopsy sections that allows for the identification of disease specific conformation variants of alpha synuclein involved in Parkinson disease and multiple proteins of the peripheral nervous system.

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