JoVE Logo
Faculty Resource Center

Sign In

Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains

DOI :

10.3791/53415-v

9:27 min

January 5th, 2016

January 5th, 2016

16,324 Views

1Reta Lila Weston Institute of Neurological Studies, UCL Institute of Neurology

Here, we present a protocol for the isolation of increasingly insoluble/aggregated alpha-synuclein (α-syn) from post-mortem human brain tissue. Through the utilization of buffers with increasing detergent strength and high-speed ultracentrifugation techniques, the variable properties of α-syn aggregation in diseased and non-diseased tissue can be examined.

Tags

Soluble Alpha synuclein

-- Views

Related Videos

article

Oral Administration of Rotenone using a Gavage and Image Analysis of Alpha-synuclein Inclusions in the Enteric Nervous System

article

Targeted Training of Ultrasonic Vocalizations in Aged and Parkinsonian Rats

article

Ex vivo Culturing of Whole, Developing Drosophila Brains

article

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

article

Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils

article

Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

article

Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation

article

Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains

article

Simultaneous Cryosectioning of Multiple Rodent Brains

article

Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved