Name: controlled cortical impact model of mouse brain injury with therapeutic transplantation of human induced pluripotent stem cell-derived neural cells
Uploaded: 2019-07-10T13:00:00
Description: Watch this Scientific Journal Video about Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells at JoVE.co

这项工作得到了神经科学和再生医学中心(CNRM,赠款号G17024014)的资助。我们感谢马希马·德万和克拉拉·塞尔布雷德在胶粘剂去除试验研究方面给予的帮助。克里斯莱·拉多姆斯基进行了初步脑损伤和细胞移植手术。USU CNRM临床前研究核心实验室的阿曼达·傅和劳拉·塔克分别就动物手术和行为测试提供了宝贵的建议。

本议定书中描述的所有实验都经过统一服务大学动物护理和使用委员会的审查和批准。
1. 颅内切除术和控制性皮质影响
<ol><li>准备受控皮质冲击装置和手术用品。<ol>
<li>装载 1 mL 滑尖注射器,带 0.5 mL 无菌盐水,用于伤口灌溉。将 25 G 针头连接到注射器以控制灌溉。</li>
		<li>在 DMSO 中制备一种加辛溶液,最终浓度为 1 mg/mL。加载第二个 1 mL 滑尖注射器,使用 0.5 mL 环孢菌素 A (CsA) ?...

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轻度CCI作为实验再生疗法测试的模型系统 CCI模型是研究皮层机械损伤后组织功能障碍机制的宝贵工具。损伤参数的可调性是该模型的一个吸引人的特征。改变Z冲击深度、速度或活动时间可以增加或减少调查人员10、25所期望的伤害严重程度。轻度CCI的损伤,如果执行正确,应导致适度的皮质细胞死亡和最小的气穴。颅骨切除术和颅瓣切除必?...

创伤性脑损伤 (TBI) 是因头部受到打击的间接机械力(旋转加速/减速或反向冲动)或物体或爆炸波的直接损坏而对大脑的习得损伤的一般术语。据估计,TBI是全世界约9%的死亡原因,每年约5000万例,每年1、2。美国疾病控制和预防中心2017年的一份报告估计,2013年,美国共有280万人次因TBI而住院和死亡。许多较温和的 TbIs 每年都会不报告。严重的TBI会导致终生的认知、运动功能和整体生活质量受损。轻度TBI的后果,特别是重复性运动相关的TBI,直到最近才被人们所认识,因为他们阴险的健康影响4,5。
临床前建模是开发TBI新的机械见解和潜在的恢复性治疗的重要组成部分。TBI受控皮质冲击(CCI)模型是机械挫伤皮层的开头模型。冲击参数可以修改,以产生CCI伤害,范围从轻度到严重6。CCI 伤害是焦点而不是扩散,如 TBI 的其他闭头模型所示。CCI 可以诱导单侧损伤,使反向皮层可以用作内部比较器。该协议演示了轻度 CCI 对皮层的一部分的特性,包括原发性躯体感觉和运动区域。这个皮质区域被选择是由于其参与感觉运动行为,其中许多行为测试可以检测损伤引起的缺陷7。TBI治疗干预引起的行为改善也可以被检测到。
TBI的一个标志是受伤地区普遍存在的神经功能障碍。受伤的神经元会经历细胞死亡,神经元网络连接中断8,9。TBI干扰了内源性干细胞的招募,导致下游行为进一步缺陷10,11。 神经干细胞和干细胞衍生细胞的移植已被探索为恢复受伤大脑功能的可能性。除了恢复受损神经回路的潜力外,移植细胞还发挥副体作用,促进TBI12的神经元生存和功能恢复。各种细胞类型已被移植临床前,以评估其恢复潜力在神经疾病模型13,14,15。最近普及的诱导多能干细胞技术16促进了许多人类干细胞系的实验应用的发展。使用hiPSC衍生细胞进行临床前测试是描述特定细胞系对人类疾病的潜在疗效的重要的第一步。该实验室已开发出将hiPSC区分为神经表型17的协议,以寻求可移植细胞,以帮助从创伤性脑损伤中恢复。
该协议的实验使用单侧CCI诱导成年小鼠的左躯体感觉和运动皮层TBI。轻度 CCI 损伤导致右前爪持续功能缺陷,用于跟踪 hiPSC 衍生神经细胞移植对功能恢复的影响。该协议中的前爪传感器运动测试是改编自布埃特及其18位同事制定的方法,之前由弗莱明和同事19演示。 该协议概述了执行实验性脑损伤、hiPS细胞治疗移植以及实验结果测量的行为和组织分析的完整工作流程。

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			<td height="20" style="height:20px;width:238px;">1 ml syringes</td>
			<td style="width:201px;">Becton Dickinson (BD)</td>
			<td style="width:123px;">&#160;309659</td>
			<td style="width:212px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">1.7 ml flip top test tubes</td>
			<td>Denville</td>
			<td style="width:123px;">C2170</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">10 microliter syringe</td>
			<td>Hamilton</td>
			<td style="width:123px;">7635-01</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">25G Precision Glide syringe needles</td>
			<td>Becton Dickinson (BD)</td>
			<td style="width:123px;">&#160;305122</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">70% ethanol</td>
			<td></td>
			<td style="width:123px;"></td>
			<td>Product of choice; varies by region</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">acetaminophen oral suspension</td>
			<td>Tylenol (Children&#39;s)</td>
			<td style="width:123px;"></td>
			<td>Dilute to 1 mg/ml in water</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">anesthetic vaporizer</td>
			<td>Vetland</td>
			<td style="width:123px;">521-11-22</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">animal handling cloth</td>
			<td></td>
			<td style="width:123px;"></td>
			<td>Purchase from department store</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">Betadine</td>
			<td>Purdue Products</td>
			<td style="width:123px;">NDC-67618-151-32</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">compressed oxygen</td>
			<td></td>
			<td style="width:123px;"></td>
			<td>Product of choice; varies by region</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">cyclosporine A</td>
			<td>Sigma-Aldrich</td>
			<td style="width:123px;">30024-100mg</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">DAB staining kit</td>
			<td>Vector Laboratories</td>
			<td style="width:123px;">SK-4100</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">dimethyl sulfoxide (DMSO)</td>
			<td>Sigma-Aldrich</td>
			<td style="width:123px;">D8418-500ml</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">DMEM</td>
			<td>Invitrogen (ThermoFisher)</td>
			<td style="width:123px;">A14430-01</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">donkey anti-mouse IgG antibody, HRP conjugated</td>
			<td>Jackson ImmunoResearch</td>
			<td style="width:123px;">715-035-151</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">electrical tape</td>
			<td>3M Corporation</td>
			<td style="width:123px;"></td>
			<td>Purchase from department store</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">fine tweezers</td>
			<td>Fine Science Tools</td>
			<td style="width:123px;">11254-20</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">forceps</td>
			<td>Fine Science Tools</td>
			<td style="width:123px;">91106-12</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">glass capillary pipettes, 1 mm OD, 0.58 mm ID</td>
			<td>World Precision Instruments</td>
			<td style="width:123px;">1B100F-3</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">High Speed Rotary Micromotor Kit</td>
			<td>Foredom Electric Co.&#160;</td>
			<td style="width:123px;">K.1070 - K.107018</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">Ideal Micro Drill Burr Set Of 5</td>
			<td>Cell Point Scientific&#160;</td>
			<td style="width:123px;">60-1000</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">Impact One Stereotaxic Impactor for CCI&#160;</td>
			<td>Leica Biosystems</td>
			<td style="width:123px;">39463920</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">isoflurane</td>
			<td>Baxter</td>
			<td style="width:123px;">NDC-10019-360-60</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">lab bench timers</td>
			<td>Fisher Scientific</td>
			<td style="width:123px;">14-649-17</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Micropipette puller</td>
			<td>MicroData Instruments, Inc.</td>
			<td style="width:123px;">PMP-102</td>
			<td>Any puller will suffice</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">Microscope cover slips</td>
			<td>Fisherbrand</td>
			<td style="width:123px;">12-545-E</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">Microscope slide mounting medium</td>
			<td></td>
			<td style="width:123px;"></td>
			<td style="width:212px;">Product of choice</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">mirror</td>
			<td></td>
			<td style="width:123px;"></td>
			<td>Purchase from department store</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">mouse anti-human nuclear antigen antibody</td>
			<td>Millipore</td>
			<td style="width:123px;">MAB1281</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">Mouse on Mouse blocking kit</td>
			<td>Vector Laboratories</td>
			<td style="width:123px;">BMK-2202</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">needle holder hemostat</td>
			<td>Fine Science Tools</td>
			<td style="width:123px;">12002-12</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">ophthalmic ointment</td>
			<td>Falcon Pharmaceuticals</td>
			<td style="width:123px;">NDC-61314-631-36</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">ophthalmic spring scissors</td>
			<td>Fine Science Tools</td>
			<td style="width:123px;">15018-10</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">plastic box</td>
			<td></td>
			<td style="width:123px;"></td>
			<td>Purchase from department store</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">plastic cylinder</td>
			<td></td>
			<td style="width:123px;"></td>
			<td>Purchase from department store</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">QSI motorized syringe pump</td>
			<td>Stoelting</td>
			<td style="width:123px;">53311</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">Removable needle compression fitting</td>
			<td>Hamilton</td>
			<td style="width:123px;">55750-01</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">small rodent stereotaxic frame</td>
			<td>Stoelting</td>
			<td style="width:123px;">51925</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">small scissors</td>
			<td>Fine Science Tools</td>
			<td style="width:123px;">14060-09</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">StemPro Accutase</td>
			<td>Invitrogen (ThermoFisher)</td>
			<td style="width:123px;">A1110501</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">Sterile alcohol prep pads</td>
			<td>Fisherbrand</td>
			<td style="width:123px;">06-669-62</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">sterile cotton swabs/Kendall Q-tips</td>
			<td>Tyco Healthcare</td>
			<td style="width:123px;">540500</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">Sterile saline</td>
			<td>Hospira</td>
			<td style="width:123px;">NDC-0409-1966-07</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">Stopwatches (2)</td>
			<td>Fisher Scientific</td>
			<td style="width:123px;">06-662-56</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">Superfrost Plus Gold microscope slides</td>
			<td>Fisherbrand</td>
			<td style="width:123px;">15-188-48</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:238px;">sutures - 5.0 silk with curved needle</td>
			<td>Oasis</td>
			<td style="width:123px;">MV-682</td>
			<td></td>
		</tr>
	</tbody>
</table>

controlled cortical impact model of mouse brain injury with therapeutic transplantation of human induced pluripotent stem cell-derived neural cells

创伤性脑损伤 (TBI) 是全世界发病率和死亡率的主要原因。TBI引起的疾病病理学从初级机械损伤进展到继发性损伤过程,包括凋亡和炎症。动物建模在探索解开损伤机制和评估潜在的神经保护疗法方面很有价值。该协议描述了焦、开头TBI的受控皮质冲击(CCI)模型。具体来说,描述了产生轻度单侧皮质损伤的参数。利用双边传感器电机集成的胶带去除试验,分析了CCI的行为后果。关于TBI病理学的实验治疗,该方案还说明了将培养细胞移植到大脑中的过程。从人类诱导多能干细胞(hiPSCs)中提取的神经细胞培养物被选择,因为他们有可能在人类TBI患者中表现出卓越的功能恢复。使用经过修饰的DAB免疫组织化学过程检测宿主小鼠脑组织中的hiPSC的慢性存活率。

颅内切除手术有助于实验性脑损伤和治疗性细胞移植:脑损伤的受控皮质冲击模型和随后的细胞移植治疗需要仔细切除上盖颅骨。颅骨切除术可以在头骨的任何背表面进行,以便对感兴趣的大脑区域进行操作。图1中的图描述了一个直径为5毫米的颅骨切除示意图,以揭示主躯体感觉和运动皮质(图1A)。在颅骨切除术后的24小时,进行了...

Watch this Scientific Journal Video about Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells at JoVE.co

Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

该协议演示了开放颅外脑损伤小鼠模型的方法,以及将培养的人类诱导多能干细胞衍生细胞移植到损伤部位的方法。对这些程序的结果的行为和组织学测试也进行了简要描述。

人类诱导多能干细胞衍生神经细胞治疗移植小鼠脑损伤控制皮质影响模型

Traumatic brain injury (TBI) is a leading cause of morbidity and mortality worldwide. Disease pathology due to TBI progresses from the primary mechanical ...

我们的方案演示了一个全面的临床前工作流程，用于测试脑损伤后人类诱导多能干细胞的疗效。这种创伤性脑损伤模型通过严格控制机械力的参数，从而提供高度的多功能性和可重复性，从而提供明确的损伤。这些程序可用于研究其他脑区域的损伤和恢复过程，移植方法可用于多种细胞类型。颅面钻孔具有挑战性，因为该技术需要手动灵巧，以掌握适当的压力、速度、深度和平滑运动。记录所有事件并广泛实践。要进行单方颅骨切除术，请确认麻醉小鼠对手趾捏没有反应，并保护小鼠在立体轴框架中。使用无菌棉签和碘溶液将抗生素眼科软膏涂抹到眼睛上，以剃光头皮区域。用70%乙醇去除碘，用装饰的手术窗帘盖住动物。在头皮上做一个1.5至2厘米的中线切口，并使用无菌棉签清洁伤口，并清除中线左侧的筋膜。使用冲击探针识别颅骨切除术位点，将 x 等于零，y 等于为 bregma 的零立体税参考点。横向调整探头到中线左侧两毫米。使用细尖手术安全标记，在假定冲击区域周围地标出一个直径为 5 毫米的圆。将冲击器从位置升起和旋转，并完成五毫米圆。将撞击器短暂地返回位置，以验证五毫米圆的准确性。然后将冲击器提升和旋转出位置，并使用配备圆尖 0.6 或 0.8 毫米毛刺钻头的高速旋转微型电机套件手动工具，以 70% 至 80% 的最大速度在头骨上打一个开孔。沿此边界内的五毫米圆周轮廓钻孔时，对头骨施加轻压，并使用钳子抬起生成的骨瓣。钻穿缝合线可能会带来额外的困难，因为骨骼被打断，需要额外的压力。血管可能密切关联，因此出血事件是可能的。要引起轻度控制的皮质撞击损伤，请用无菌酒精准备垫清洁冲击探针，将冲击探针移回暴露皮层的位置。降低探头，直到它接触杜拉母体表面，并标记此位置为 z 等于零。退出活塞并移动到 z 等于负一毫米，并释放活塞以冲击皮层。立即抬起活塞，将手臂移出位置，用大量盐水灌溉皮层，并使用简单的中断缝合和 5-0 丝缝来关闭头皮切口。然后通过皮下注射将每公斤环孢素 A 中 10 毫克，放入擦伤中，将鼠标放入清洁的预热后笼中，进行监测，直到完全恢复。大约24小时后，颅骨切除术，把鼠标放回立体轴框架。用装饰的手术窗帘盖住鼠标。用无菌盐水将切口部位拉下，以清洁部位并松开缝合线。使用 70% 乙醇浸泡棉签轻轻消毒切口部位，并使用细钳子和眼科剪刀去除缝合线。然后用丰富的无菌盐水灌溉手术和颅骨切除术。在细胞培养生物安全罩中，轻轻旋转或敲击细胞管，以确保细胞均匀悬浮。使用微管通过柱塞端将大约 7.5 微升的细胞加载到汉密尔顿注射器中。将注射器以 120 度角按住，柱塞端朝下，插入柱塞，注意不要在悬架和柱塞尖端之间引入气泡。将垫片组件连接到移液器针，然后将针头连接到注射器。推动柱塞将细胞悬浮液移入移液器针，将注射器连接到立体税注射器泵。推进柱塞，确保注射器泵组件正常工作，并将针头移入注射坐标。将针尖对齐到啤酒，然后将 x 和 y 坐标设置为零。将针头翻过颅骨切除术移动到两毫米的横向和负一毫米的后部，然后触摸针头尖到杜拉母体表面。稍微抬起针头，然后在针头接触的位置的 dura 母体中做一个小切口。将立体轴坐标设置为 z 等于零，然后推柱塞以确认细胞悬浮液在将针头引入大脑之前流动充足。将针头引入大脑的深度为 z 等于负 1.4 毫米，将移植物放在深层皮层的灰质/白色物质边界处。启动注射器泵，在 15 分钟内注入细胞悬浮液。使用长工作距离显微镜监测细胞悬浮液流出，在注射过程中用无菌盐水灌溉手术部位，以保持组织水化。当所有细胞都已交付后，慢慢提取移植针头，用额外的盐水灌溉手术部位，然后用新的缝合线关闭切口。然后提供术后护理，如证明。对于胶带去除测试，首先使用一把小剃刀将黄色和红色的电气胶带切割成三条五毫米的胶带，在光滑的玻璃表面。在测试前将小鼠带进行为测试室至少 30 分钟，并使用处理布将小鼠用颈部和背部的擦伤约束小鼠，使小鼠将前爪从身体和头部拉开。使用钳子在每个前爪的植物表面放置粘合条，并使用细腻且一致的手指压力将胶带固定到爪子上。快速将鼠标放入塑料缸中，当鼠标在塑料测试盒上全部使用四只爪子时，启动两个秒表。然后使用两个秒表记录左爪通知、左爪拆卸、右爪通知和右爪拆卸的延迟。在这个试验实验中，仅颅切除术、控制皮质撞击损伤和天真小鼠的前肢功能进行了比较。接受手术的小鼠在手术后立即出现一到三天的胶粘剂刺激，并且从伊皮拉特前爪去除胶粘剂时出现暂时性增加的延迟。然而，与术后第28天天真的老鼠相比，受控制皮质撞击的小鼠在手术前与损伤的逆向性相比，在运动性能上表现出显著的缺陷。人类核抗原小鼠大脑部分的免疫性损伤表明，人体细胞移植物可以清楚地与SHAM损伤和控制皮质冲击大脑中的宿主组织区分开来。在处理培养的人体细胞和处理与啮齿动物或免疫抑制药物接触的注射器针头时，必须遵守标准的实验室安全程序。脑组织部分可以分析神经炎症标记的表达，因为它是有价值的知道移植物如何影响宿主组织损伤反应，反之亦然。我们惊讶地发现，在受伤后的行为结果中，性别差异微妙的。我们现在正在调查性是否在脑损伤的神经免疫反应中起一定的作用。稳定的手，耐心和实践是发展良好的颅骨切除术技术的关键。不当颅骨切除术造成的附带损害可能会损害您想要靶向细胞移植的区域。

Research

JoVE Journal

Developmental Biology

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research

培养人多能性和神经干细胞的基本和临床前研究一个封闭的细胞培养系统

This paper describes how to use a custom manufactured, commercially available enclosed cell culture system for basic and preclinical research. Biosafety ...

科研

发育生物学

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

诱导多能干细胞的快速神经元分化有关微电极阵列测量网络活动

Neurons derived from human induced Pluripotent Stem Cells (hiPSCs) provide a promising new tool for studying neurological disorders. In the past decade, ...

Pluripotent Stem Cell Derived Cardiac Cells for Myocardial Repair

多能干细胞衍生的心肌修复心肌细胞

Human induced pluripotent stem cells (hiPSCs) must be fully differentiated into specific cell types before administration, but conventional protocols for ...

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

分化软骨细胞与外周血源性人诱导多能干细胞

In this study, we used peripheral blood cells (PBCs) as seed cells to produce chondrocytes via induced pluripotent stem cells (iPSCs) in an ...

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

人诱导多能干细胞的前脑型脑 Organoids 标准化和可再生性的生成

The human cortex is highly expanded and exhibits a complex structure with specific functional areas, providing higher brain function, such as cognition. ...

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

人诱导多潜能干细胞衍生肝细胞的药物发现方法

The ability to differentiate human induced pluripotent stem cells (iPSCs) into hepatocyte-like cells (HLCs) provides new opportunities to study inborn ...

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

诱导多潜能干细胞源性肝细胞的家族性高胆固醇血症人肝嵌合小鼠模型

Familial hypercholesterolemia (FH) is mostly caused by low-density lipoprotein receptor (LDLR) mutations and results in an increased risk of early-onset ...

Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)

Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells hiPSC-ECs

微流控法评价人诱导的多能干细胞内皮细胞 (hipsc-ec) 对白细胞粘附的评价

Endothelial cells (ECs) are essential for the regulation of inflammatory responses by either limiting or facilitating leukocyte recruitment into affected ...

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

从脐带血源性多能干细胞中提取三维皮肤有机细胞

The skin is the body&#8217;s largest organ and has many functions. The skin acts as a physical barrier and protector of the body and regulates bodily ...

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

人诱导多能干细胞体外生成体细胞衍生物的研究

In response to signals such as WNTs, bone morphogenetic proteins (BMPs), and sonic hedgehog (SHH) secreted from surrounding tissues, somites (SMs) give ...