Preparation of Animal, Procurement of Cardiac Tissue, and Dissociation of Heart Tissue
3:28
Preparation of the Crude Cell Mixture for Fluorescence Activating Cell Sorting (FACS)
6:34
Purification of Pericytes from Crude Cell Mixture Using FACS
8:18
Results: Analysis of Crude Cells and Characterization of Primary Isolated Cardiac Pericytes
9:55
Conclusion
Vi har optimeret en protokol til at isolere og rense murine hjerte pericytes for grundforskning og undersøgelse af deres biologi og terapeutiske potentiale.