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DOI :
10.3791/59615-v
•
10:00 min
June 20th, 2019
Chapters
0:04
Title
0:57
Preparation of Buffers, System, and Sample, and Loading of Sample
3:42
Preparation of the MALS Software and the FPLC Software
5:49
Analysis of SEC-MALS BSA Data
8:04
Results: SEC-MALS Analysis
9:16
Conclusion
该协议描述了尺寸排除色谱与多角度光散射(SEC-MALS)的结合,用于对溶液中的蛋白质和复合物进行绝对表征。SEC-MALS 确定纯蛋白质、原生寡聚物、异质复合物和修饰性蛋白质(如糖蛋白)的分子量和大小。
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