Isolamento e cultura delle cellule endoteliali aortiche primarie dai suini in miniatura
August 14th, 2019
•Yanli Zhao1,2,3, Chengjiang Zhao4, David K.C. Cooper5, Ying Lu2, Kewang Luo6, Huiyun Wang3, Pengfei Chen3, Changchun Zeng3, Shaodong Luan1, Lisha Mou2, Hanchao Gao1,2,3
1Department of Nephrology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 2Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, 3Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 4Department of Endocrinology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 5Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, 6People's Hospital of Longhua
Viene descritto un metodo enzimatico efficace per l'isolamento delle cellule endoteliali sui porcili primari (pAEC) dai suini in miniatura. I pAEC primari isolati possono essere utilizzati per studiare la risposta immunitaria e di coagulazione nello xenotrapianto.
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved