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DOI :
10.3791/59678-v
•
10:47 min
November 6th, 2019
Chapters
0:04
Title
1:12
Preparation of Device, Gel, and Coating
2:42
Cell Seeding/Microvessel Culture
4:37
Study Angiogenic Sprouting
5:49
Study Anastomosis and Sprout Stabilization
6:50
Fixation, Staining, and Imaging
8:55
Results: Cell Seeding and 3D Sprouting of iPSC-EC
10:13
Conclusion
この方法は、標準化されたマイクロ流体プラットフォームにおける40の浸透した3Dマイクロ容器としてiPSC由来内皮細胞の培養を記述する。このプラットフォームは、アナストモシスや、スケーラブルで高スループットな方法での血管新芽の安定化を含む、3Dでの勾配駆動血管新生芽芽の研究を可能にします。
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