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DOI :
10.3791/59683-v
July 1st, 2019
Chapters
0:04
Title
0:45
Mouse Eye Enucleation, Eye Cup Dissection, and Embedding
3:08
Sectioning Using a Cryostat
3:51
Results: Immunohistochemistry of Mouse Retinal Cryo-sections
5:01
Conclusion
本報告では、免疫組織化学(IHC)のための凍結マウス網膜切片を準備するための包括的な方法について説明する。 記載された方法は、眼後カップの解剖、パラホルムアルデヒド固定、最適切断温度(OCT)培媒体および組織配向への埋め込み、断断および免疫染色を含む。
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