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DOI :
10.3791/59695-v
May 21st, 2019
Chapters
0:04
Title
0:44
Nuclear and Cytoplasmic Extraction
2:31
Synaptic Fraction Collection
3:45
Proteasome Activity Assay
6:36
Quantification of Linkage-specific Protein Ubiquitination
7:34
Results: Differential Subcellular Changes in Proteasome Activity and Protein Polyubiquitination in the Rat Amygdala Following Learning
9:13
Conclusion
このプロトコルは、げっ歯類の脳の異なる細胞区画におけるユビキチンプロテアソーム系(UPS)活性を効率的に定量するように設計されている。ユーザーは、同じ動物の核、細胞質およびシナプス画のUPS機能を調べることができ、これらの複雑な分析を行うために必要な時間と動物の数を減らすことができます。
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