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DOI :
10.3791/59706-v
•
9:57 min
August 12th, 2019
Chapters
0:04
Title
0:47
Preparation of the Monocyte Chemoattractant Protein (MCP)-1-loaded Growth Factor-reduced Basement Membrane-derived Solution
1:40
Basement Membrane-derived Solution Injection and Gel Plug Harvest
3:29
Basement Membrane-derived Gel Plug Digestion
4:43
Single-cell Western Blot (scWB) Analysis
6:38
scWB Chip Stripping
7:35
Results: Representative Basement Membrane-derived Gel Plug Cell Recruitment Analyses
9:22
Conclusion
在这里,我们提出一个协议,以量化小鼠模型中血液单核细胞的化学活性,评估营养、药理和遗传干预对血液单核细胞的影响,并描述在小鼠模型中产生的血单核细胞使用单核细胞-化学吸引蛋白-1(MCP-1)加载的基底膜衍生凝胶塞的小鼠模型。
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