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Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

DOI :

10.3791/59714-v

June 16th, 2019

June 16th, 2019

24,545 Views

1Section for Cell Biology and Physiology, Department of Biology, Faculty of Science, University of Copenhagen

Here, we present several simple methods for evaluating viability and death in 3D cancer cell spheroids, which mimic the physico-chemical gradients of in vivo tumors much better than the 2D culture. The spheroid model, therefore, allows evaluation of the cancer drug efficacy with improved translation to in vivo conditions.

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Cell Viability

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