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Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures

DOI :

10.3791/60357-v

December 13th, 2019

December 13th, 2019

8,671 Views

1Department of Urology, College of Medicine, University of Illinois at Chicago, 2Department of Pathology, College of Medicine, University of Illinois at Chicago, 3University of Illinois Cancer Center, College of Medicine, University of Illinois at Chicago

Using human primary prostate epithelial cells, we report a novel biomarker-free method of functional characterization of stem-like cells by a spheroid-based label-retention assay. A step-by-step protocol is described for BrdU, CFSE, or Far Red 2D cell labeling; three-dimensional spheroid formation; label-retaining stem-like cell identification by immunocytochemistry; and isolation by FACS.

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Stem like Cells

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