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DOI :
10.3791/59749-v
July 12th, 2019
Chapters
0:04
Title
0:47
Adult Mouse Hind Limb Distal P3 Amputation
2:01
Digit Collection and Tissue Preparation
2:47
Immunostaining for Blastema Formation and Intramembranous Ossification
4:30
Sequential In Vivo Microcomputed Tomography (microCT) Imaging
5:50
Reconstructed Bone Image Analysis
8:15
Results: Representative Regenerative Bone Analyses
8:47
Conclusion
ここでは、哺乳類のブラセマ形成と内皮形成を調べ、蛍光免疫組織化学と順次生体内マイクロコンピュータ断層撮影により分析した成体マウス末端ファランクス切断のプロトコルを提示する。
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