Name: fluorescence angiography for evaluation of aneurysm perfusion and parent artery patency in rat and rabbit aneurysm models
Uploaded: 2019-07-24T14:00:00
Description: Watch this Scientific Journal Video about Fluorescence Angiography for Evaluation of Aneurysm Perfusion and Parent Artery Patency in Rat and Rabbit Aneurysm Models at JoVE.com

这项研究部分得到了瑞士坎顿斯皮特塔尔·阿劳的研究资助。

啮齿动物被安置在动物护理设施中,实验由瑞士伯尔尼大学动物福利委员会(BE 108/16)和(BE65/16)审查和批准。所有动物都按照标准实验室饮食,免费获得食物和水。所有动物实验都是在仔细考虑3R(替换、减少和优化)的情况下进行的。包括10只新西兰雌性白兔和48只雄性威斯塔鼠。已严格遵守了《到达准则》8。
注:在10只兔子身上,通过缝合供体兔子的胡萝卜动脉上的...

<ol>
	<li>Kakucs, C., Florian, I. A., Ungureanu, G., Florian, I. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fluorescein+Angiography+in+Intracranial+Aneurysm+Surgery:+A+Helpful+Method+to+Evaluate+the+Security+of+Clipping+and+Observe+Blood+Flow.">Fluorescein Angiography in Intracranial Aneurysm Surgery: A Helpful Method to Evaluate the Security of Clipping and Observe Blood Flow.</a> World Neurosurgery. 105, 406-411 (2017).</li><li>Ajiboye, N., Chalouhi, N., Starke, R. M., Zanaty, M., Bell, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Unruptured+Cerebral+Aneurysms:+Evaluation+and+Management.">Unruptured Cerebral Aneurysms: Evaluation and Management.</a> ScientificWorldJournal. 2015, 954954 (2015).</li><li>Suzuki, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Confirmation+of+blood+flow+in+perforating+arteries+using+fluorescein+cerebral+angiography+during+aneurysm+surgery.">Confirmation of blood flow in perforating arteries using fluorescein cerebral angiography during aneurysm surgery.</a> Journal of Neurosurgery. 107 (1), 68-73 (2007).</li><li>Gruter, B. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fluorescence+Video+Angiography+for+Evaluation+of+Dynamic+Perfusion+Status+in+an+Aneurysm+Preclinical+Experimental+Setting.">Fluorescence Video Angiography for Evaluation of Dynamic Perfusion Status in an Aneurysm Preclinical Experimental Setting.</a> Operative Neurosurgery. , (2019).</li><li>Raabe, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prospective+evaluation+of+surgical+microscope-integrated+intraoperative+near-infrared+indocyanine+green+videoangiography+during+aneurysm+surgery.">Prospective evaluation of surgical microscope-integrated intraoperative near-infrared indocyanine green videoangiography during aneurysm surgery.</a> Journal of Neurosurgery. 103 (6), 982-989 (2005).</li><li>Riva, M., Amin-Hanjani, S., Giussani, C., De Witte, O., Bruneau, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Indocyanine+Green+Videoangiography+in+Aneurysm+Surgery:+Systematic+Review+and+Meta-Analysis.">Indocyanine Green Videoangiography in Aneurysm Surgery: Systematic Review and Meta-Analysis.</a> Neurosurgery. , (2017).</li><li>Kuroda, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intra-arterial+injection+fluorescein+videoangiography+in+aneurysm+surgery.">Intra-arterial injection fluorescein videoangiography in aneurysm surgery.</a> Neurosurgery. 72, 141-150 (2013).</li><li>Kilkenny, C., Browne, W. J., Cuthill, I. C., Emerson, M., Altman, D. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improving+Bioscience+Research+Reporting:+The+ARRIVE+Guidelines+for+Reporting+Animal+Research.">Improving Bioscience Research Reporting: The ARRIVE Guidelines for Reporting Animal Research.</a> PLOS Biology. 8 (6), 1000412 (2010).</li><li>Marbacher, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Helsinki+rat+microsurgical+sidewall+aneurysm+model.">The Helsinki rat microsurgical sidewall aneurysm model.</a> Journal of Visualized Experiments. (92), e51071 (2014).</li><li>Marbacher, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Complex+bilobular,+bisaccular,+and+broad-neck+microsurgical+aneurysm+formation+in+the+rabbit+bifurcation+model+for+the+study+of+upcoming+endovascular+techniques.">Complex bilobular, bisaccular, and broad-neck microsurgical aneurysm formation in the rabbit bifurcation model for the study of upcoming endovascular techniques.</a> American Journal of Neuroradiology. 32 (4), 772-777 (2011).</li><li>Shurey, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+rat+model+in+microsurgery+education:+classical+exercises+and+new+horizons.">The rat model in microsurgery education: classical exercises and new horizons.</a> Archives of Plastic Surgery. 41 (3), 201-208 (2014).</li><li>Foster, S. D., Lyons, M. S., Runyan, C. M., Otten, E. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+mimic+of+soft+tissue+infection:+intra-arterial+injection+drug+use+producing+hand+swelling+and+digital+ischemia.">A mimic of soft tissue infection: intra-arterial injection drug use producing hand swelling and digital ischemia.</a> World Journal of Emergency Medicine. 6 (3), 233-236 (2015).</li><li>Flower, R. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Injection+technique+for+indocyanine+green+and+sodium+fluorescein+dye+angiography+of+the+eye.">Injection technique for indocyanine green and sodium fluorescein dye angiography of the eye.</a> Investigative Ophthalmology &amp; Visual Science. 12 (12), 881-895 (1973).</li><li>Yoshioka, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Advantage+of+microscope+integrated+for+both+indocyanine+green+and+fluorescein+videoangiography+on+aneurysmal+surgery:+case+report.">Advantage of microscope integrated for both indocyanine green and fluorescein videoangiography on aneurysmal surgery: case report.</a> Neurologia medico-chirurgica (Tokyo). 54 (3), 192-195 (2014).</li><li>Ichikawa, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+and+Clinical+Experience+with+a+Simple+Device+for+Performing+Intraoperative+Fluorescein+Fluorescence+Cerebral+Angiography:+Technical+Notes.">Development of and Clinical Experience with a Simple Device for Performing Intraoperative Fluorescein Fluorescence Cerebral Angiography: Technical Notes.</a> Neurologia medico-chirurgica. 56 (3), 141-149 (2016).</li><li>Alander, J. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+review+of+indocyanine+green+fluorescent+imaging+in+surgery.">A review of indocyanine green fluorescent imaging in surgery.</a> International Journal of Biomedical Imaging. 2012, 940585 (2012).</li><li>Lane, B., Bohnstedt, B. N., Cohen-Gadol, A. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+prospective+comparative+study+of+microscope-integrated+intraoperative+fluorescein+and+indocyanine+videoangiography+for+clip+ligation+of+complex+cerebral+aneurysms.">A prospective comparative study of microscope-integrated intraoperative fluorescein and indocyanine videoangiography for clip ligation of complex cerebral aneurysms.</a> Journal of Neurosurgery. 122 (3), 618-626 (2015).</li><li>Blair, N. P., Evans, M. A., Lesar, T. S., Zeimer, R. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fluorescein+and+fluorescein+glucuronide+pharmacokinetics+after+intravenous+injection.">Fluorescein and fluorescein glucuronide pharmacokinetics after intravenous injection.</a> Investigative Ophthalmology &amp; Visual Science. 27 (7), 1107-1114 (1986).</li><li>Hillmann, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+optical+imaging+of+physiological+responses+to+photostimulation+in+human+photoreceptors.">In vivo optical imaging of physiological responses to photostimulation in human photoreceptors.</a> Proceedings of the National Academy of Sciences of the United States of America. 113 (46), 13138-13143 (2016).</li><li>Golby, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Image-Guided Neurosurgery. , (2015).</li></ol>

FVA 是一种有前途的简单方法,用于检测啮齿动物中的容器,并且可以使用商业设备和现成设备进行。FVA 可在任何需要手术中评估容器完整性的手术中实施,因为容器需要首先进行适当的解剖。
作者更喜欢静脉注射,而不是动脉注射,因为意外事件的风险较低,如感染,缺血症和隔间综合征12。静脉注射使可靠,空间有限,高度浓缩染色,并要求小染料剂量13,14。<sup class="x...

完全动脉瘤消解和母动脉完整性的证据在动脉瘤手术中至关重要。有几个选项来确认父动脉闭塞和动脉闭塞,如多普勒血管造影,常规脑血管造影(DSA),计算机断层扫描血管造影(CTA)或磁共振血管造影(MRA)1, 2.然而,这些方法既昂贵又耗时,在实验室环境中往往不可用。此外,它们可能有相关的副作用,如辐射暴露或需要额外的镇静实验动物,以避免运动艺术品。
随着越来越多的新的血管内设备出现,连续需要对此类设备进行临床前测试。然而,这些研究往往依赖于验尸分析(例如,宏观病理学和组织学),并且缺乏关于动态灌注的信息。此外,对于研究人员来说,在实验外科手术中获得即时可靠的信息可能至关重要。荧光血管造影是一种经济高效且易于执行可视化技术1,3,4。
因此,英青绿(ICG)视频血管造影术常用于临床神经外科手术,并已广泛研究5,6。荧光视频血管造影 (FVA) 是一种替代技术,其附加优势是创建荧光信号,该信号位于人类视觉的波长范围内,因此肉眼无需扩展光谱红外摄像机即可看到7.氟化物视频血管造影在临床脑血管手术中较少使用,在实验环境中关于FVA的报告很少1,4。
本报告旨在论证FVA在大鼠和兔前脑血管研究中的应用的可行性和范围。

<table border="0" cellpadding="0" cellspacing="0" style="width:651px;" width="651">
	<colgroup>
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		<col />
		<col />
	</colgroup>
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">For rabbits</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Aluminium foil</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Animal shaver</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Black tape</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Blue filter</td>
			<td style="width:149px;">Thorlabs MF475-35</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Body warm plate</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Camera</td>
			<td style="width:149px;">Sony NEX-5R</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Catheter</td>
			<td style="width:149px;">22G Vasofix Safety</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Disinfictant</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Fluorescein sodium</td>
			<td style="width:149px;">Fluorescein Faure 10%</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Glas plate</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Green filter</td>
			<td style="width:149px;">Thorlabs MF539-43</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Incontinence pad</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Infusion pump</td>
			<td style="width:149px;">Perfusor Secura</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Ketamine hydrochloride</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td>any generic products</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Needle</td>
			<td style="width:149px;">25G</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Oxygen</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Ringer&#39;s Solution</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Sterile sheets</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Surgical instruments</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td>micro forceps, micro scissor, blunt surgical scissor</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Surgical microscope</td>
			<td colspan="3">OPMI, Carl Zeiss AG, Oberkochen, Germany</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Syringe 2ml, 5ml, 50ml</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Tape</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Three-way-stopcock</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Torch light</td>
			<td></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Xylazin</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td>any generic products</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">For rats</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Aluminium foil</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Animal shaver</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Black tape</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Blue filter</td>
			<td style="width:149px;">Thorlabs MF475-35</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Body warm plate</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Camera</td>
			<td style="width:149px;">Sony NEX-5R</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Disinfictant</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Fluorescein sodium</td>
			<td style="width:149px;">Fluorescein Faure 10%</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Green filter</td>
			<td style="width:149px;">Thorlabs MF539-43</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Incontinence pad</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Isoflurane</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Ketamine hydrochloride</td>
			<td></td>
			<td></td>
			<td>any generic products</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Medetomidine hydrochloride</td>
			<td></td>
			<td></td>
			<td>any generic products</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Needle</td>
			<td style="width:149px;">25G</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Oxygen</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Plate</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Ringer&#39;s Solution</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Sterile sheets</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Surgical instruments</td>
			<td></td>
			<td></td>
			<td>micro forceps, micro scissor, blunt surgical scissor</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Surgical microscope</td>
			<td colspan="3">OPMI, Carl Zeiss AG, Oberkochen, Germany</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Syringe 2ml, 5ml</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Tape</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Torch light</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

fluorescence angiography for evaluation of aneurysm perfusion and parent artery patency in rat and rabbit aneurysm models

脑动脉瘤治疗的重点是实现完全闭塞,以及保持父母动脉的血流。氟西因钠和丁氰酸绿分别用于观察血流和血管灌注状态。本研究的目的是应用FVA来验证在兔子和大鼠诱导侧壁动脉瘤后实时血流、血管灌注和动脉瘤的闭塞,并验证这些物种的程序。
通过在捐赠兔的胡萝卜动脉上缝合一个去细胞化的动脉袋,在10只兔子身上制造了20个侧壁动脉瘤。此外,在48只大鼠中制造了48个显微外科侧壁动脉瘤。在创建后一个月的随访期间,对母动脉/动脉瘤复合物进行解剖,并使用静脉荧光管(10%,1 mL)注射,通过兔子的耳静脉导管插入和大鼠的股骨静脉催化进行FVA注射。动脉瘤随后被收获,并宏观评估。
从宏观上看,兔子16个动脉瘤中的14个表明没有残留的母动脉灌注,完全被遮挡的发光体,然而11(79%)被FVA检测到。由于技术问题,排除了四个动脉瘤。在大鼠中,在48例中,有25例在宏观上观察到残留动脉瘤灌注。在23个没有灌注宏观证据的病例中,FVA确认了22例动脉瘤的发病率(96%)。没有与FVA相关的不良事件。荧光辛易于应用,无需特殊设备。它是一种安全、极其有效的方法,用于评估在实验环境中与兔子和大鼠的动脉完整性和动脉瘤的亲动脉完整性/残留灌注。使用荧光辛作为造影剂的FVA似乎能有效控制动脉瘤和基础血管的锥度,甚至可以适应旁路手术。

在手术期间监测心率和血压。兔子的平均心率为193/分钟,大鼠为196/分钟。兔子的体重在3.05-4.18公斤,老鼠的体重为335-690克。
我们能够在十只兔子中的八只(图1)中执行FVA。由于技术困难,两只兔子的四次动脉瘤检查没有用相机记录下来。没有关于大鼠FVA的技术困难报告。然而,FVA不能在一只大鼠中执行,因为难以刺穿股骨静脉。
在8只兔子...

Watch this Scientific Journal Video about Fluorescence Angiography for Evaluation of Aneurysm Perfusion and Parent Artery Patency in Rat and Rabbit Aneurysm Models at JoVE.com

Fluorescence Angiography for Evaluation of Aneurysm Perfusion and Parent Artery Patency in Rat and Rabbit Aneurysm Models

荧光血管造影用于评估大鼠和兔动脉瘤模型中的动脉灌注和父动脉鉴赏

我们使用基于荧光的荧光视频血管造影(FVA),提出一种方案,以有效评估大鼠和兔子的动脉灌注和侧壁动脉瘤的血管鉴口。阳性预测值为92.6%,是一种简单但非常有效、经济的方法,无需特殊设备。

Brain aneurysm treatment focuses on achieving complete occlusion, as well as preserving blood flow in the parent artery. Fluorescein sodium and ...

该方法是一种可靠、简单的技术，用于直观地可视化动脉瘤和血管中的实时血液流动，可用于不同的动物模型。该技术的主要优点是，它便于在手术过程中血液流动的可视化，而无需任何昂贵的设备或广泛的手术。在开始该程序之前，使用黑色胶带固定绿色带通滤波器，以充当手电筒上的激励过滤器。并检查手电筒只发出蓝光。为摄像机配备绿色带通滤波器，以用作发射光滤镜。现在只有绿灯才能通过。然后，用消毒液对工作空间进行消毒，用无菌窗帘盖住桌子，防止污染。在兔子中进行荧光素视频造影或FVA，确认麻醉动物在将兔子定位在超前对疼痛刺激没有反应。剃颈膜肌瘤。将相机聚焦在解剖动脉上，使用铝箔覆盖一个含有100毫克/毫升荧光素钠的5毫升注射器。尽可能关灯，静脉注射0.3毫升/千克荧光素钠到导管注射口。接下来，用0.5毫升的盐水溶液冲洗注射器，以确保所有染料都已经注射。并立即用改良的手电筒照亮手术场。一旦灯亮着，开始使用修改后的相机进行拍摄。血流应在注射后几秒钟内可见。用于血管造影后血管的宏观分析，可切除随发动物中的动脉瘤和母动脉复合物，或通过观察动脉瘤的填充和/或去除临时夹后旁路进行视觉确认。在这项具有代表性的研究中，FVA能够在10只兔子中8只进行。在8只兔子中确定的16例动脉瘤中，有2例动脉瘤表明母动脉持续灌注，而FVA则发现5例有残留灌注。14只兔动脉瘤在宏观上没有残留灌注，而随后使用FVA检测出11种。在48只大鼠中，有23只在宏观上观察到残余灌注，其他25只大鼠没有余量灌注的宏观迹象。23个没有宏观灌注的动脉瘤中的22例随后使用FVA得到确认。总体而言，27例兔鼠合并病例中，有25例可以确诊，结果预测值为92.6%，灵敏度为100%，特异性为94.1%。此过程可用于任何动物模型，以评估任何可见容器中的血流。

Research

JoVE Journal

Neuroscience

Focal Cerebral Ischemia Model by Endovascular Suture Occlusion of the Middle Cerebral Artery in the Rat

焦距脑缺血模型大鼠大脑中动脉的血管内缝合闭塞

Stroke is the leading cause of disability and the third leading cause of death in adults worldwide1. In human stroke, there exists a highly variable ...

科研

神经科学

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

横向扩散和培养的神经元膜蛋白的胞吐评估使用荧光恢复和荧光损失漂白

Membrane proteins such as receptors and ion channels undergo active trafficking in neurons, which are highly polarised and morphologically complex. This ...

Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue

在大鼠脑组织用荧光活化细胞分选，以检查细胞类型特异性基因表达

The brain is comprised of four primary cell types including neurons, astrocytes, microglia and oligodendrocytes. Though they are not the most abundant ...

Evaluation of Bioenergetic Function in Cerebral Vascular Endothelial Cells

在脑血管内皮细胞生物能量功能的评价

The integrity of the blood-brain-barrier (BBB) is critical to prevent brain injury. Cerebral vascular endothelial (CVE) cells are one of the cell types ...

Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy

非人类灵长类动物脑组织的制备预嵌入免疫组化和电子显微镜

Despite all the technological advances at the light microscopy&#160;level, electron microscopy remains the only tool in neuroscience to examine and ...

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

脑血流动力学和的光散射特性的同步评估<em&gt;体内</em&gt;大鼠脑使用多光谱漫反射成像

The simultaneous evaluation of cerebral hemodynamics and the light scattering properties of in vivo rat brain tissue is demonstrated using a conventional ...

3D Kinematic Analysis for the Functional Evaluation in the Rat Model of Sciatic Nerve Crush Injury

坐骨神经粉碎损伤大鼠模型中功能评估的3D运动学分析

Compared to the Sciatic Functional Index (SFI), kinematic analysis is a more reliable and sensitive method for performing functional evaluations of ...

Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit

兔动脉袋显微外科分叉动脉瘤模型

Endovascular treatment for intracranial aneurysms gained importance over the past decades, consequently there is an increased need of testing endovascular ...

Semi-Quantitative Determination of Dopaminergic Neuron Density in the Substantia Nigra of Rodent Models using Automated Image Analysis

使用自动图像分析对啮齿动物模型的亚斯坦蒂娅尼格拉多巴明神经元密度进行半定量测定

Estimation of the number of dopaminergic neurons in the substantia nigra is a key method in pre-clinical Parkinson's disease research. Currently, unbiased ...

Motor Dual-Tasks for Gait Analysis and Evaluation in Post-Stroke Patients

用于中风后患者步态分析和评估的运动双任务

Eighteen stroke patients were recruited for this study involving the evaluation of cognition and walking ability and multitask gait analysis. Multitask ...