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DOI :
10.3791/59836-v
August 9th, 2019
Chapters
0:04
Title
0:46
Human Umbilical Cord Blood Mononuclear Cell (MNC) Isolation
2:45
CD34+ Cell Isolation from MNCs
4:55
Flow Cytometric Analysis of Hematopoietic Stem and Progenitor Cell (HSPC) Populations
5:59
CD34+ HSPC Myeloid Differentiation
8:06
Results: Representative Characterization of Freshly-isolated and Differentiated HSPCs
9:47
Conclusion
ここでは、4つの骨髄系統に対する臍帯血由来CD34+造血幹細胞および前駆細胞の免疫発食特性特性およびサイトカイン誘導分化のためのプロトコルを提示する。このプロトコルの応用には、CD34+細胞の骨髄性分化に対する骨髄疾患変異または小分子の影響に関する調査が含まれる。
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