الجينات المباشرة ضرب من Axolotl الحبل الشوكي الخلايا الجذعية العصبية عن طريق الكهربائي من المجمعات CAS9 البروتين-gRNA

1:16

CAS9-gRNA Ribonucleoprotein Mixture and Agarose Plate Preparation

2:33

Electroporator Configuration and Glass Microinjection Capillary Loading

4:09

Pneumatic Pump Configuration

4:37

CAS9-gRNA Ribonucleoprotein Mixture Injection

6:05

Electroporation

6:45

Knock-Out Efficiency Assessment

7:25

Results: Representative Knock-Out Effects in Axolotl Spinal Cord Neural Stem Cells

8:33

Conclusion