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DOI :
10.3791/59884-v
•
8:30 min
January 31st, 2020
Chapters
0:04
Introduction
1:45
Dissection and Enzymatic Dissociation of Mucosa-free DSM Pieces
3:28
Voltage-step Induced Cation Currents from DSM Cells Using Amphotericin-B Perforated Whole-cell Voltage Patch-clamp Technique
5:52
Results: Investigation of Ion Channel Properties in Isolated DSM Cells
7:07
Conclusion
我们描述了一种从人类膀胱标本中制备单一新分离的分离性肌细胞的方法,采用两步酶程序。获得可行的DSM细胞可以通过各种单细胞技术进行研究,包括所述的amphotericin-B贴片-钳电生理学,以揭示生理和药理特性。
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