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DOI :
10.3791/59906-v
•
8:53 min
July 15th, 2019
Chapters
0:04
Title
0:56
Pre-plating of the Cryopreserved hiPSC-CM for Maturation
1:35
Multiwell MEA Plate Sterilization and Coating
2:57
hiPSC-CM Dissociation and Plating on Multiwell MEA Plate
4:09
hiPSC-CM Electroporation and Signal Acquisition
5:06
Data Segmentation and Analysis
6:40
Results: Action Potential Recordings of hiPSC-CMs
8:21
Conclusion
本文包含一套用于开发人类诱导多能干细胞衍生心肌细胞(hiPSC-CM)网络的协议,这些网络在多孔MEA板上培养,可逆电化细胞膜进行作用电势测量。高通量记录在几天内从同一单元站点重复获取。
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