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DOI :
10.3791/59924-v
February 15th, 2020
Chapters
0:00
Introduction
1:36
Ultraviolet C (UV-C) Radiation Exposure to Mouse Primary Ocular Cells
3:43
Preparation of the Living-Cell Staining Media
4:58
Live Cell Staining of UV-C Exposed Mouse Primary Ocular Cells
5:51
Viewing of DCFDA (ROS), Hoechst and PI Stained Cells
7:40
Results
11:57
Conclusion
该协议演示了从小鼠眼部表面细胞同时检测活原培养物中的活性氧物种(ROS)、活细胞和死细胞。2',7'-二氯氟乙酸酸酯,碘化钠和Hoechst染色分别用于评估ROS,死细胞和活细胞,然后成像和分析。
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