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DOI :
10.3791/59924-v
February 15th, 2020
Chapters
0:00
Introduction
1:36
Ultraviolet C (UV-C) Radiation Exposure to Mouse Primary Ocular Cells
3:43
Preparation of the Living-Cell Staining Media
4:58
Live Cell Staining of UV-C Exposed Mouse Primary Ocular Cells
5:51
Viewing of DCFDA (ROS), Hoechst and PI Stained Cells
7:40
Results
11:57
Conclusion
このプロトコルは、マウスの眼表面細胞からの活性一次培養における活性酸素種(ROS)、生細胞、および死細胞の同時検出を実証する。2',7'-ジクロロフルオレセインセテート、ヨウ化プロピジウム、ヘーチスト染色を用いてROS、死細胞、生細胞をそれぞれ評価し、続いてイメージングと分析を行います。
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