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DOI :
10.3791/59956-v
•
5:41 min
January 28th, 2020
Chapters
0:04
Title
0:40
ISH-stained Embryo Imaging and DNA Extraction
2:26
Image Analysis with Fiji Software
3:53
Results: Parallel Image Quantitation and Genotyping Protocol
5:13
Conclusion
基因编辑技术使研究人员能够相对轻松地生成斑马鱼突变体来研究基因功能。在这里,我们提供了一个指南,以执行平行胚胎基因分型和定量的原位杂交信号在斑马鱼。这种无偏的方法在基于原位杂交的表型分析中提供了更高的精度。
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