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DOI :
10.3791/59977-v
October 3rd, 2019
Chapters
0:04
Title
0:46
Generation and Purification of Lentivirus Particles
3:08
Isolation and Transduction of Lineage-negative Cells from Mouse Bone Marrow
5:23
Transplantation of Transduced Cells into Lethally Irradiated Mice
5:39
Evaluating the Chimerism of Peripheral Blood
6:19
Results: Lentiviral Transduction of Mouse Bone Marrow Lineage-negative Cells
7:23
Conclusion
CRISPR/Cas9システムによるマウス造血幹細胞および前駆細胞(HSPC)の高効率ゲノム編集のためのプロトコルは、造血系特異的遺伝子改変を用いたマウスモデルシステムを迅速に開発する。
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