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DOI :
10.3791/59977-v
October 3rd, 2019
Chapters
0:04
Title
0:46
Generation and Purification of Lentivirus Particles
3:08
Isolation and Transduction of Lineage-negative Cells from Mouse Bone Marrow
5:23
Transplantation of Transduced Cells into Lethally Irradiated Mice
5:39
Evaluating the Chimerism of Peripheral Blood
6:19
Results: Lentiviral Transduction of Mouse Bone Marrow Lineage-negative Cells
7:23
Conclusion
설명된 것은 CRISPR/Cas9 시스템에 의한 뮤린 조혈 줄기 및 전구 세포(HSPC)의 고효율 게놈 편집을 위한 프로토콜로 조혈 시스템 별 유전자 수정을 통해 마우스 모델 시스템을 신속하게 개발합니다.
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