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DOI :
10.3791/59992-v
•
7:59 min
October 4th, 2019
Chapters
0:04
Title
0:37
Diterpenoid Metabolite Production and Transformation in E. coli
2:14
Inoculation Culture Preparation
2:59
Co-Expression Cultures Preparation and Induction
4:34
Metabolite Separation and Purification
5:55
Results: Representative Diterpenoid Enzymatic Production and Purification Analyses
7:02
Conclusion
ここでは、エシェリヒア大腸菌またはニコチアナ・ベンタミアナにおける生合成酵素の組み合わせ発現を通じて、ジテルペノイド代謝物を製造および精製するためのプロトコルを簡単に提示し、クロマトグラフィー製品を提供します。浄化。得られた代謝産物は、分子構造特性解析、酵素機能研究、生理活性アッセイを含む様々な研究に適しています。
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