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DOI :
10.3791/60029-v
August 21st, 2019
Chapters
0:04
Title
0:59
Quantitative Multiplex Immunoprecipitation (Day 1)
2:19
Running the Assay (Day 2)
4:40
Data Analysis
6:10
Positive Hits and Visualization
6:57
Results: Representative Protein Interaction Network Dynamic Quantification
7:20
Conclusion
定量多重免疫沈殿(QMI)は、2つのサンプル間の標的タンパク質相互作用の豊富さの違いを敏感に検出するためにフローサイトメトリーを使用します。QMIは、少量の生体材料を用いて行うことができ、遺伝子組み換えタグを必要とせず、以前に定義されたタンパク質相互作用ネットワークに適応させることができる。
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