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DOI :
10.3791/60060-v
September 20th, 2019
Chapters
0:04
Title
0:50
Isolation of CD4+ T Cells from Spleens and Lungs of OVA-challenged Mice
4:25
Ex vivo Transmigration Procedure
6:39
Quantification of Ex vivo Transmigration
8:06
Results: Assessment of Lymphocyte Migration under Various Conditions
9:42
Conclusion
このプロトコルでは、リンパ球は、多孔質膜によって下部チャンバから分離された移行系の上部チャンバーに配置される。ケモカインは底室に添加され、ケモカイン勾配に沿って活発な移動を誘導する。48時間後、リンパ球は、移行を定量するために両方の部屋でカウントされます。
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