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DOI :
10.3791/60063-v
September 17th, 2019
Chapters
0:05
Title
0:57
Chemical Synthesis of the Hydrazine Derivative of a Fc-III Peptide
2:31
Cleavage of the SUMO Tag
4:09
Assembling of DCAF1 by Native Chemical Ligation
7:06
Mass Spectrometry and ELISA Assay
8:19
Results: Native Chemical Ligation and ELISA Assay
9:03
Conclusion
抗原ペプチドおよびFc-III模倣体(DCAF)の二重機能コンジュゲートの開発は、有害な抗体の排除のために新規である。ここでは、デングウイルス感染時に抗体依存性増強効果を排除するために4G2抗体を選択的にブロックできるDCAF1分子の合成に関する詳細なプロトコルについて説明する。
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