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DOI :
10.3791/60068-v
September 22nd, 2019
Chapters
0:04
Title
1:30
Preparation of Salmonella Typhimurium △AroA Cultures for Oral Gavage of Mice
3:16
Assessment of Salmonella Burdens in Tissues
4:36
Picrosirius Red Staining and Quantification of Collagen
5:43
Results: Chronic Salmonella Infection Induced Intestinal Fibrosis
6:26
Conclusion
このプロトコルは、トランスムラール炎症および線維症を含むクローン病の主要な病理学的特徴に似たサルモネラ駆動腸線維症のマウスモデルを記述する。この方法は、C57Bl/6遺伝的背景に維持された変異マウスを使用して線維性転帰を変化させる宿主因子を評価するために使用することができる。
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