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DOI :
10.3791/60095-v
April 19th, 2020
Chapters
0:05
Introduction
1:14
Cyrosectioning
1:54
Fluorescence Immunolabelling
3:55
Fluorescence Image Acquisition and Automated Fluorescence Image Analysis
4:55
Data Analysis
6:24
Results: Representative Murine Hepatocyte Nuclear Ploidy Estimation
8:03
Conclusion
フローサイトメトリーを必要としない固定/凍結保存組織サンプル内で、肝細胞数および核プロイドの変化を測定するための堅牢で費用対効果の高い柔軟な方法を提示します。私たちのアプローチは、肝臓の損傷や病気の進行を追跡するのに理想的な肝臓細胞学の強力なサンプル全体の署名を提供します。
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