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9.3K Views
•
09:59 min
August 13th, 2019
DOI :
10.3791/60125-v
Chapters
0:04
Title
0:59
Reproductive System Dissection and Cannulation
2:15
Basal Tone Mechanical Testing
5:55
Passive Mechanical Testing
7:54
Results: Representative Biaxial Basal Tone and Passive Murine Reproductive System Testing
9:06
Conclusion
Transcript
该协议研究生殖器官的双轴力学特性,阐明平滑肌细胞和被动基质的贡献。这种技术保持器官几何形状和原生平滑肌肉细胞到矩阵相互作用,允许器官受受生理范围的压力和轴向延伸。在生理相关负荷下研究器官双轴功能可能有助于更好地了解生殖病理的病因。
对子宫颈和阴道进行偏见测试有助于深入了解女性健康和生物力学领域。类似的方法用于研究血管,食道和大肠。当使用新器官时,可能需要时间确定技术细节,例如如何最好地缝合组织或如何确定卸载的长度。
要从实验动物中收集生殖系统,请将鼠标放在解剖显微镜
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Summary
该协议使用市售的压力子宫内科系统对鼠阴道和子宫颈进行压力阴唇测试。利用有钙和无钙的介质,在估计的生理条件下,对器官分离出平滑肌细胞(SMC)基底色和被动细胞外基质(ECM)的贡献。
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