Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/60130-v
•
9:16 min
September 1st, 2019
Chapters
0:04
Title
1:16
crRNA Design and Preparation of Cas9/gRNA RNP Complexes
2:16
Electroporation of RNP Complexes into OCI-AML3 Cells
3:12
Screening and Selection of Cell Clones with Biallelic Deletions
5:51
Functional Analyses of Silencer Deletions with Real-time Quantitative RT-PCR
7:18
Results: RUNX1 Intronic Silencer Deletion
8:16
Conclusion
組み立て済みのCas9/ガイドRNAリボヌクレオタンパク質複合体の直接送達は、血行細胞におけるゲノム編集のための迅速かつ効率的な手段である。ここでは、このアプローチを用いてRUNX1イントロニックサイレンサーを削除し、OCI-AML3白血病細胞における転写応答を調べる。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved