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DOI :
10.3791/60171-v
October 6th, 2019
Chapters
0:04
Title
0:55
Porcine Eye Preparation
1:35
Dissection and Incubation
3:43
Microscopy and Endothelium Examination
5:11
Corneal Endothelium Image Processing
6:15
Corneal Endothelial Cell Density and Morphological Parameter Analysis
6:49
Results: Representative Corneal Button Culture Analyses
8:12
Conclusion
ここでは、ブタ分割角膜ボタンの調製および栽培のためのステップバイステップのプロトコルが提示される。このオルガノ典型的に栽培された臓器培養モデルは、ヒトドナー角膜に匹敵する15日以内の細胞死亡率を示すので、有毒なデキストランを添加することなく非ヒト角膜の長期培養を可能にする最初のモデルを表す。
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