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DOI :
10.3791/60223-v
•
10:38 min
November 22nd, 2019
Chapters
0:05
Title
0:52
Plating Sorted Prostate Epithelial Cells into Primary Mouse Organoid Culture
3:29
Extracting Protein Lysate from Prostate Organoids for Western Blot Analysis
4:50
Fixing and Staining Prostate Organoids for Immunohistochemical Analysis by Whole-Mount Confocal Microscopy
6:44
Tissue Clearing and Mounting of the Stained Prostate Organoids for Whole-mount Confocal Microscopy
7:57
Results: Analysis of Lineage Marker Expression in Prostate Organoids by Western Blot and Whole-Mount Confocal Microscopy
9:44
Conclusion
マウス前立腺オルガノイドは、分化を調節するメカニズムを評価する有望な文脈を表す。本論文では、前立腺オルガノイドを確立するための改善されたアプローチについて述べ、(1)オルガノイドからタンパク質リサートを採取する方法、および(2)全マウント共焦点顕微鏡のためのオルガノイドを修正および染色する方法を紹介する。
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