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DOI :
10.3791/60271-v
•
10:22 min
September 17th, 2019
Chapters
0:04
Title
0:58
Animal Dissection and Preparation of Germ Cell Suspension
4:50
Separation of Germ Cells Through the Discontinuous BSA Gradient
6:02
Collection of Enriched Germ Cell Fractions
6:52
Analysis of Cell Fractions
8:06
Results: MDR Cell Enrichment
9:29
Conclusion
这里介绍的是一个协议,用于使用标准实验室设备,使用不连续的牛血清白蛋白密度梯度,丰富白细胞、圆形精子和从成年小鼠睾人身上拉长精子。
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