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DOI :
10.3791/60271-v
•
10:22 min
September 17th, 2019
Chapters
0:04
Title
0:58
Animal Dissection and Preparation of Germ Cell Suspension
4:50
Separation of Germ Cells Through the Discontinuous BSA Gradient
6:02
Collection of Enriched Germ Cell Fractions
6:52
Analysis of Cell Fractions
8:06
Results: MDR Cell Enrichment
9:29
Conclusion
ここでは、標準的な実験装置を用いて不連続な牛血清アルブミン密度勾配を用いて、成人マウス精巣からパチテン精子細胞、円形精子細胞、および細長い精子を濃縮するためのプロトコルを提示する。
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