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DOI :
10.3791/60297-v
January 8th, 2020
Chapters
0:05
Title
1:24
Detection of Double-crossover Recombinants of P. aeruginosa
2:24
Confirmation of Gene Deletion with Colony PCR
3:07
Visualization of Bioluminescence-labeled Bacteria in Mice
5:42
Inoculation of Animals with Bacterial Strains
6:28
Results: Evaluation of Attenuated P. aeruginosa Strain
7:24
Conclusion
ここでは、市販品に対する米国食品医薬品局(FDA)承認の大腸菌と比較して、緑膿菌の遺伝子組み換え株の減衰を評価する感染のマウスモデルの簡単かつ再現性の高いプロトコルについて説明する。
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